WebFLAG Purification. Recombinant/Fusion Tag Protein Purification. Description. General description. The FLAG epitope, is an eight amino acid protein with an enterokinase-cleavage site. The rabbit Anti-FLAG polyclonal affinity antibody ANTI-FLAG recognizes the FLAG epitope located on FLAG fusion proteins. This antibody reacts with N-terminal, N ... WebThe FLAG ® HA Tandem Affinity Purification Kit is designed for the isolation of high purity FLAG-HA dual-tagged fusion proteins from complex matrix, such as cell lysates and …
FLAG Immunoprecipitation Kit for recombinant FLAG tag proteins.
WebFigure 1. Schematic representation of the FLAG® HA Tandem Affinity Purification system. The whole procedure involves six simple steps: 1) Prepare the starting material that … WebFLAG is an affinity tag widely used for rapid and highly specific one-step protein purification. Native elution of protein from anti-FLAG antibody resins allows the identification of protein and nucleic acid binding partners and functional analysis using biochemical activity assays. daily weekly monthly cleaning checklist
Anti-FLAG® M2 Magnetic Beads - Sigma-Aldrich
WebThe FLAG ® HA Tandem Affinity Purification Kit is designed for the isolation of high purity FLAG-HA dual-tagged fusion proteins from complex matrix, such as cell lysates and tissue homogenates. The kit is particularly suitable for isolation of protein complexes using TAP (Tandem Affinity Purification) technology. Sutitable for mass spectrometry analysis … WebAnti-FLAG ® M2 magnetic beads (M8823) were thoroughly resuspended and 20 µL of suspension (including 10 µL of packed resin) were aliquoted into several 1.5 mL … WebFor purification of N-terminal FLAG fusion proteins. Since binding is Ca 2+-dependent, proteins can be eluted with a buffer containing EDTA, as well as by the standard methods using either FLAG peptide or glycine-HCl buffer, pH 3.ANTI-FLAG M1 does not bind to Met-FLAG fusion proteins, so this resin is not appropriate for purifying unprocessed, … daily weekly homes